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Nihon Pharmaceutical CO
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GRiSP Research Solutions
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Image Search Results
Journal: Protein and Peptide Letters
Article Title: Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives
doi: 10.2174/0929866528666210805120715
Figure Lengend Snippet: Effect of additives on recombinant GFPuv production using auto-induction. ( A ) Final OD 600 and final pH of the culture media. OD 600 , grown without additives ( white bar ); OD 600 , grown with additives ( gray bar ); pH, grown without additives ( open square ); pH, grown with additives ( open circle ). ( B ) Relative fluorescence per cell density. ( C ) Relative total fluorescence. The fluorescence values obtained without ( white bar ) or with ( gray bar ) additives are shown in the panels.Transformed E. coli cells were grown with six different culture media without additives, the results for which are shown on the left of each medium condition. Independently, four cultures with additives and two control cultures without additives were carried out, the results for which are shown on the right of each condition. The two independent cultures of each condition were subjected to statistical analysis using a Student’s t-test: *, P <0.05; NS, not significant. One-way analysis of variance followed by Dunnett's test was performed for comparison of culture media. a, P <0.05 compared to ON medium ( left ); b, P <0.05 compared to ON medium ( right ). Error bar indicates the standard deviation value. Statistical significance was identified in pH results for all conditions except TB-2 medium (not shown for clarity). Abbreviations: LB, Luria-Bertani; LBM, LB Broth Miller; OD 600 , optical density at 600 nm; ON, Overnight Express™ Instant TB; TB, Terrific Broth.
Article Snippet: Two types of
Techniques: Recombinant, Fluorescence, Transformation Assay, Control, Comparison, Standard Deviation
Journal: Protein and Peptide Letters
Article Title: Boosting Auto-Induction of Recombinant Proteins in Escherichia coli with Glucose and Lactose Additives
doi: 10.2174/0929866528666210805120715
Figure Lengend Snippet: Effect of additives on recombinant oANG production using auto-induction. ( A ) Final OD 600 and final pH of the culture medium. OD 600 , grown without the additives ( white bar ); OD 600 , grown with the additives ( gray bar ); pH, grown without the additives ( open square ); pH, grown with the additives ( open circle ). ( B ) oANG amount per cell density. ( C ) Total oANG amount. The amounts obtained without ( white bar ) or with ( gray bar ) additives are shown in the panels. The cultivation design, as well as, graphical representation of results are the same as in the case of GFPuv. The details of statistics analysis are same as those described in the legend for Figure . Statistical significance was identified in pH results for all conditions except TB-1 and TB-2 media (not shown). LB, Luria-Bertani; LBM, LB Broth Miller; oANG, ovine angiotensinogen; OD 600 , optical density at 600 nm; ON, Overnight Express™ Instant TB; TB, Terrific Broth.
Article Snippet: Two types of
Techniques: Recombinant